Quantitative Proteomics

Quantitative Proteomics

Quantitative Proteomics is a scientific technique for determining the amount of protein in a sample, which may then be used to compare diseased and healthy people. It also provides information on sample differences. Isotopic labelling of proteins or peptides can be differentiated using mass spectrometry. Other life science domains such as genomics, kinemics, transcriptomics, and metabolomics are increasingly using broad-scope analysis, and the quantitative Proteomic approach is in line with that. Quantitative Dot Blot (QDB) Electrospray Ionization 2DE-DIGE Mass Spectroscopy Optimizing LC-Ms/MS for Quantitative Proteomics.

Quantification using spectrophotometry

Spectrophotometric methods can be used to determine the concentration of a specific protein in a sample. A spectrophotometer may be used to measure the OD at 280 nm of a protein, which can then be used in conjunction with a standard curve assay to quantify the content of Tryptophan, Tyrosine, and Phenylalanine. This method, however, is not the most accurate because protein composition varies widely, and this method would be unable to quantify proteins that do not contain the aforementioned amino acids. Due to the likelihood of nucleic acid contamination, this approach is also imprecise. Biuret, Lowry, BCA, and other more accurate spectrophotometric techniques for protein quantification.

 

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